How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Since its invention in the 1980s, polymerase chain reaction (PCR) opened the door for applications such as genetic engineering, genotyping, and sequencing, and has become a fundamental part of ...

Once the concept of PCR popped into Kary Mullis’ head in 1983 it wasn’t long before scientists realized they had a revolutionary technology in their hands. Because now they could take the smallest ...

Discover the principles behind Countable PCR, how it compares with established molecular quantification methods, and what direct RNA counting could mean for research and clinical applications.